Get more Mail & Guardian
Subscribe or Login

‘Knockout mice’ win scientist trio the Nobel Prize

Mario Capecchi and Oliver Smithies of the United States and Martin Evans of Britain won the Nobel Prize for Medicine on Monday for their work in creating ”knockout mice”, the 21st-century testbed for biomedical research.

The trio were honoured for discovering how to manipulate genetically mouse embryonic stem cells, leading to lab rodents that replicate human disease, the Nobel jury said in its citation.

Their ”ground-breaking discoveries concerning embryonic stem cells and DNA recombination in mammals … led to the creation of an immensely powerful technology”, the committee said.

The discovery is technically called gene targeting, but is commonly known as gene ”knockout”.

Engineered mice provide researchers with a lab model that yields insights into the fundamentals of diseases ranging from Alzheimer’s to cancer and the response to new drugs, the jury said.

Thanks to their work, scientists can now determine the role of specific genes, a breakthrough that has ”revolutionised life science”, it said. ”Gene targeting in mice has pervaded all fields of biomedicine. Its impact on the understanding of gene function and its benefits to mankind will continue to increase over many years to come.”

To date, more than 10 000 mice genes — approximately half of the genes in the mammalian genome — have been knocked out.

Disabling, or knocking out, a gene is a two-step process. The first is to snip out a functioning gene from the animal’s genome, using chemical ”scissors” such as an enzyme. The next is to replace that gene with the modified one — the gene whose flaws will cause the disease to be studied.

The big challenge is getting this introduced stretch of DNA to find the corresponding slot in the chromosome and then fit in snugly.

Little more than two decades ago, the prevailing wisdom was that the task was impossible in mammalian cells and that the DNA would insert itself in the chromosome almost randomly.

The Nobel laureates found a way to do this. In the 1980s, Evans isolated mice embryonic stem cells — the all-purpose master cells whose manipulation could create in theory any mutation of choice.

In the meantime, Capecchi and Smithies, working independently of each other, found a way to target genes by a technique called ”homologous recombination”.

”Homologous” means that the introduced DNA sequence lines up with its mirror target sequence in the mouse chromosome, while ”recombination” means the incoming and target sequences break and then rejoin.

”The award is very exciting and particularly appropriate,” observed Steve Brown, a leading scientist at the Mammalian Genetics Unit of Britain’s Medical Research Council. ”Our ability to knock out — to lose the function — of a gene in the mouse genome has been absolutely critical in understanding the genetic basis of human disease in all areas.”

Evans, born in 1941 and who has been knighted by Queen Elizabeth II, is the director of the School of Biosciences and professor of mammalian genetics at Cardiff University in Britain.

”He is a world leader in mammalian genetics and his research has undoubtedly increased our understanding of human diseases,” said Martin Rees, president of Britain’s de facto academy of sciences, the Royal Society.

Italian-born Capecchi, who celebrated his 70th birthday on Saturday, is a human genetics and biology professor at the University of Utah, while British-born Smithies (82) is a professor of pathology at the University of North Carolina.

Last year, the prize went to two US scientists, Andrew Fire and Craig Mello, for discovering how to silence malfunctioning genes, a breakthrough that could lead to an era of new therapies to reverse crippling disease.

The medicine prize is the first award to be announced in this year’s Nobel season.

The physics prize is to be announced on Tuesday followed by the chemistry prize on Wednesday. The literature prize will be announced on Thursday and the peace prize on Friday. The economics prize wraps up the season on October 15.

The Nobels, founded by Swedish industrialist Alfred Nobel, were first awarded in 1901. Laureates receive a gold medal, a diploma and 10-million Swedish kronor ($1,53-million), which can be split between up to three winners per prize.

The award ceremony will take place in Stockholm on December 10. — Sapa-AFP

Subscribe to the M&G

Thanks for enjoying the Mail & Guardian, we’re proud of our 36 year history, throughout which we have delivered to readers the most important, unbiased stories in South Africa. Good journalism costs, though, and right from our very first edition we’ve relied on reader subscriptions to protect our independence.

Digital subscribers get access to all of our award-winning journalism, including premium features, as well as exclusive events, newsletters, webinars and the cryptic crossword. Click here to find out how to join them.

Pia Ohlin
Guest Author

Related stories


If you’re reading this, you clearly have great taste

If you haven’t already, you can subscribe to the Mail & Guardian for less than the cost of a cup of coffee a week, and get more great reads.

Already a subscriber? Sign in here


Subscribers only

Mbeki tells ANC that land without compensation goes against the...

‘This would be a very serious disincentive to investment,’ says Thabo Mbeki in a document arguing that the ANC should not proceed with the Constitutional amendment of section 25

Micro-hydropower lights up an Eastern Cape village

There is hidden potential for small hydropower plants in South Africa

More top stories

SIU freezes R22-million in Digital Vibes accounts

The Special Investigating Unit said it would ask the tribunal to declare the health department’s contract with the company unlawful

Life-saving free train travel offered to domestic abuse victims in...

A pioneering railway scheme in the UK is helping domestic violence victims to escape their abusers by providing them with free travel to reach refuge

Oral submissions to inquiry on local government elections start next...

The hearings will be open to the media and the public, under strict level-three regulations

Education employees queue for Covid jabs, but some may have...

People who have had Covid-19 in the past 30 days or who have had a flu shot in the past 14 days will be vaccinated at a later date

press releases

Loading latest Press Releases…