South African National Roads Agency Limited (Sanral) spokesperson Vusi Mona told the Cape Times on Wednesday almost three-quarters of these jobs would go to lower-paid workers.
After construction, about 600 direct jobs would be generated a year.
"The upgrade of the N1-N2 Winelands route will be of great benefit to all communities in the region and will not impact negatively on poorer communities," he said.
"Sanral views the upgrading of the road network as a major infrastructural investment which will lead to increased job opportunities and will also boost the economy in the region."
The Democratic Alliance announced on Monday that it would mobilise residents against tolling.
Inequalities between 'black and white areas'
Congress of South African Trade Unions' (Cosatu) provincial secretary Tony Ehrenreich also said he would oppose the project.
Ehrenreich reportedly said Cosatu would not support running an anti-tolling campaign with the Democratic Alliance unless it publicly stated it would use funds to remove huge inequalities between "black and white areas".
Provincial premier Helen Zille said it was hypocritical of Ehrenreich to oppose the project when the ANC caucus in the city council, led by Ehrenreich, voted against the city's decision to fight tolls.
Ehrenreich said: "I can't say I recall that happening. We would never do that. We have always been against tolling".
The DA on Monday called on voters to punish the ANC in the polls next year, if the Sanral goes ahead with the proposed toll roads on the N1 and N2 highways that link Cape Town to the rest of the Western Cape and the country.
Treat next year’s election as a referendum
The DA said it will also continue to fight the tolling of the highways in the courts and is prepared to go to the Constitutional Court in its fight.
Provincial minister of transport Robin Carlisle told journalists on Monday the DA will call on the people of the Western Cape to treat next year’s election as a referendum on these two toll roads.
“Every vote for the ANC in the April 2014 would thus be a vote for the toll road and every vote against the ANC regardless for which party, will be a vote against the toll road,” said Carlisle.
“We in the DA call on the voters of the Western Cape to show the ANC the real teeth of a functioning democracy. We must ensure that every voter in the Western Cape understands exactly what that toll road means to them before the day of the elections.” – Sapa